| File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig\Results\Saliva IgG Luitpold d22 061813.rbx | |||||||||||||||||
| Analyte: IgG (77) | |||||||||||||||||
| Acquisition Date: 18-Jun-2013, 12:13 PM | |||||||||||||||||
| Reader Serial Number: LX10008241401 | |||||||||||||||||
| Plate ID: | |||||||||||||||||
| RP1 PMT (Volts): 648.60 | |||||||||||||||||
| RP1 Target: 17026 | |||||||||||||||||
| Analyte | Type | Well | Outlier | Description | FI | FI - Bkgd | Std Dev | Std Err | %CV | Obs Conc | Exp Conc | (Obs/Exp) * 100 | Conc in Range | Bead Count | Sampling Errors | ||
| IgG (77) | B | A1,B1,C1,D1 | 0 | 167.5 | 167.5 | 4.36 | 2.18 | 2.6 | |||||||||
| IgG (77) | S1 | E1,F1 | 2 | Standard 1 | 25546 | 25378.5 | 0 | 0 | 0 | 782.29 | 1000 | 78 | 782.29 | ||||
| IgG (77) | S2 | G1,H1 | 2 | Standard 2 | 25259 | 25091.5 | 0 | 0 | 0 | 554.54 | 500 | 111 | 554.54 | ||||
| IgG (77) | S3 | A2,B2 | 0 | Standard 3 | 23988.3 | 23820.8 | 69.65 | 49.25 | 0.29 | 271.11 | 250 | 108 | 271.11 | ||||
| IgG (77) | S4 | C2,D2 | 0 | Standard 4 | 20304.8 | 20137.3 | 242.18 | 171.25 | 1.19 | 116.82 | 125 | 93 | 116.82 | ||||
| IgG (77) | S5 | E2,F2 | 0 | Standard 5 | 16107.3 | 15939.8 | 278.25 | 196.75 | 1.73 | 65.37 | 62.5 | 105 | 65.37 | ||||
| IgG (77) | S6 | G2,H2 | 0 | Standard 6 | 10118 | 9950.5 | 11.31 | 8 | 0.11 | 30.52 | 31.25 | 98 | 30.52 | ||||
| IgG (77) | S7 | A3,B3 | 0 | Standard 7 | 6107.5 | 5940 | 23.33 | 16.5 | 0.38 | 15.8 | 15.63 | 101 | 15.8 | ||||
| IgG (77) | S8 | C3,D3 | 2 | Standard 8 | 3373 | 3205.5 | 0 | 0 | 0 | 7.79 | 7.81 | 100 | 7.79 | ||||
| IgG (77) | X1 | E3,F3 | 0 | 4661 | 798.5 | 631 | 7.78 | 5.5 | 0.97 | *1505.60 | *** | ||||||
| IgG (77) | X2 | G3,H3 | 0 | 4331 | 3882.5 | 3715 | 0.71 | 0.5 | 0.02 | 9183.45 | 9183.45 | ||||||
| IgG (77) | X3 | A4,B4 | 0 | 4326 | 1836.5 | 1669 | 38.89 | 27.5 | 2.12 | *3873.23 | *** | 3873.23 | |||||
| IgG (77) | X4 | C4,D4 | 0 | 4651 | 2337 | 2169.5 | 40.31 | 28.5 | 1.72 | *5101.87 | *** | 5101.87 | |||||
| IgG (77) | X5 | E4,F4 | 0 | 4653 | 2399.3 | 2231.8 | 10.96 | 7.75 | 0.46 | *5258.05 | *** | 5258.05 | |||||
| IgG (77) | X6 | G4,H4 | 0 | 4654 | 1871.8 | 1704.3 | 32.17 | 22.75 | 1.72 | *3958.13 | *** | 3958.13 | |||||
| IgG (77) | X7 | A5,B5 | 0 | 4655 | 958.5 | 791 | 13.44 | 9.5 | 1.4 | *1851.19 | *** | 1851.19 | |||||
| IgG (77) | X8 | C5,D5 | 0 | 4657 | 1537.5 | 1370 | 20.51 | 14.5 | 1.33 | *3163.78 | *** | 3163.78 | |||||
| IgG (77) | X9 | E5,F5 | 0 | 4658 | 2236.5 | 2069 | 24.75 | 17.5 | 1.11 | *4851.26 | *** | 4851.26 | |||||
| IgG (77) | X10 | G5,H5 | 0 | 4659 | 1612 | 1444.5 | 41.01 | 29 | 2.54 | *3338.71 | *** | 3338.71 | |||||
| IgG (77) | X11 | A6,B6 | 0 | 4660 | 2656.3 | 2488.8 | 54.09 | 38.25 | 2.04 | *5910.39 | *** | 5910.39 | |||||
| IgG (77) | X12 | C6,D6 | 0 | 4662 | 1266.3 | 1098.8 | 9.55 | 6.75 | 0.75 | *2537.91 | *** | 2537.91 | |||||
| IgG (77) | X13 | E6,F6 | 0 | 4663 | 1703.8 | 1536.3 | 13.79 | 9.75 | 0.81 | *3555.85 | *** | 3555.85 | |||||
| IgG (77) | X14 | G6,H6 | 0 | 4665 | 1177 | 1009.5 | 12.73 | 9 | 1.08 | *2336.02 | *** | 2336.02 | |||||
| IgG (77) | X15 | A7,B7 | 0 | 4666 | 1651.8 | 1484.3 | 298.75 | 211.25 | 18.09 | *3432.56 | *** | 3432.56 | |||||
| IgG (77) | X16 | C7,D7 | 0 | 4667 | 1559.8 | 1392.3 | 44.19 | 31.25 | 2.83 | *3215.89 | *** | 3215.89 | |||||
| IgG (77) | X17 | E7,F7 | 0 | 4669 | 787 | 619.5 | 17.68 | 12.5 | 2.25 | *1481.10 | *** | 1481.1 | |||||
| IgG (77) | X18 | G7,H7 | 0 | 4670 | 2948.3 | 2780.8 | 30.76 | 21.75 | 1.04 | *6665.98 | *** | 6665.98 | |||||
| IgG (77) | X19 | A8,B8 | 0 | 4671 | 1958.3 | 1790.8 | 45.61 | 32.25 | 2.33 | *4167.54 | *** | 4167.54 | |||||
| IgG (77) | X20 | C8,D8 | 0 | 4672 | 3988.8 | 3821.3 | 67.53 | 47.75 | 1.69 | 9479.31 | 9479.31 | 9479.31 | |||||
| Analyte | Type | Well | Outlier | Description | FI | FI - Bkgd | Std Dev | Std Err | %CV | Obs Conc | Exp Conc | (Obs/Exp) * 100 | Conc in Range | Bead Count | Sampling Errors | ||
| IgG (77) | B | A1 | 0 | 162 | 162 | 257 | |||||||||||
| IgG (77) | B | B1 | 0 | 171 | 171 | 197 | |||||||||||
| IgG (77) | B | C1 | 0 | 166 | 166 | 201 | |||||||||||
| IgG (77) | B | D1 | 0 | 171 | 171 | 245 | |||||||||||
| IgG (77) | S1 | E1 | 0 | 25546 | 25378.5 | 782.29 | 1000 | 78 | 782.29 | 288 | |||||||
| IgG (77) | S1 | F1 | 1 | 25291 | 25123.5 | 1000 | 250 | ||||||||||
| IgG (77) | S2 | G1 | 0 | 25259 | 25091.5 | 554.54 | 500 | 111 | 554.54 | 262 | |||||||
| IgG (77) | S2 | H1 | 1 | 25447.5 | 25280 | 500 | 258 | ||||||||||
| IgG (77) | S3 | A2 | 0 | 24037.5 | 23870 | 276.13 | 250 | 110 | 276.13 | 252 | |||||||
| IgG (77) | S3 | B2 | 0 | 23939 | 23771.5 | 266.28 | 250 | 107 | 266.28 | 240 | |||||||
| IgG (77) | S4 | C2 | 0 | 20133.5 | 19966 | 113.67 | 125 | 91 | 113.67 | 234 | |||||||
| IgG (77) | S4 | D2 | 0 | 20476 | 20308.5 | 120.13 | 125 | 96 | 120.13 | 283 | |||||||
| IgG (77) | S5 | E2 | 0 | 15910.5 | 15743 | 63.78 | 62.5 | 102 | 63.78 | 260 | |||||||
| IgG (77) | S5 | F2 | 0 | 16304 | 16136.5 | 67 | 62.5 | 107 | 67 | 235 | |||||||
| IgG (77) | S6 | G2 | 0 | 10126 | 9958.5 | 30.55 | 31.25 | 98 | 30.55 | 218 | |||||||
| IgG (77) | S6 | H2 | 0 | 10110 | 9942.5 | 30.48 | 31.25 | 98 | 30.48 | 193 | |||||||
| IgG (77) | S7 | A3 | 0 | 6091 | 5923.5 | 15.75 | 15.63 | 101 | 15.75 | 269 | |||||||
| IgG (77) | S7 | B3 | 0 | 6124 | 5956.5 | 15.86 | 15.63 | 101 | 15.86 | 285 | |||||||
| IgG (77) | S8 | C3 | 0 | 3373 | 3205.5 | 7.79 | 7.81 | 100 | 7.79 | 221 | |||||||
| IgG (77) | S8 | D3 | 1 | 3044 | 2876.5 | 7.81 | 251 | ||||||||||
| IgG (77) | X1 | E3 | 0 | 793 | 625.5 | *1493.87 | *** | 252 | |||||||||
| IgG (77) | X1 | F3 | 0 | 804 | 636.5 | *1517.33 | *** | 210 | |||||||||
| IgG (77) | X2 | G3 | 0 | 3883 | 3715.5 | 9184.84 | 9184.84 | 234 | |||||||||
| IgG (77) | X2 | H3 | 0 | 3882 | 3714.5 | 9182.06 | 9182.06 | 239 | |||||||||
| IgG (77) | X3 | A4 | 0 | 1864 | 1696.5 | *3939.44 | *** | 247 | |||||||||
| IgG (77) | X3 | B4 | 0 | 1809 | 1641.5 | *3807.18 | *** | 252 | |||||||||
| IgG (77) | X4 | C4 | 0 | 2365.5 | 2198 | *5173.28 | *** | 222 | |||||||||
| IgG (77) | X4 | D4 | 0 | 2308.5 | 2141 | *5030.61 | *** | 206 | |||||||||
| IgG (77) | X5 | E4 | 0 | 2391.5 | 2224 | *5238.57 | *** | 252 | |||||||||
| IgG (77) | X5 | F4 | 0 | 2407 | 2239.5 | *5277.54 | *** | 189 | |||||||||
| IgG (77) | X6 | G4 | 0 | 1894.5 | 1727 | *4013.06 | *** | 252 | |||||||||
| IgG (77) | X6 | H4 | 0 | 1849 | 1681.5 | *3903.31 | *** | 209 | |||||||||
| IgG (77) | X7 | A5 | 0 | 968 | 800.5 | *1871.97 | *** | 233 | |||||||||
| IgG (77) | X7 | B5 | 0 | 949 | 781.5 | *1830.43 | *** | 217 | |||||||||
| IgG (77) | X8 | C5 | 0 | 1552 | 1384.5 | *3197.73 | *** | 223 | |||||||||
| IgG (77) | X8 | D5 | 0 | 1523 | 1355.5 | *3129.88 | *** | 201 | |||||||||
| IgG (77) | X9 | E5 | 0 | 2254 | 2086.5 | *4894.76 | *** | 368 | |||||||||
| IgG (77) | X9 | F5 | 0 | 2219 | 2051.5 | *4807.81 | *** | 171 | |||||||||
| IgG (77) | X10 | G5 | 0 | 1583 | 1415.5 | *3270.47 | *** | 228 | |||||||||
| IgG (77) | X10 | H5 | 0 | 1641 | 1473.5 | *3407.15 | *** | 257 | |||||||||
| IgG (77) | X11 | A6 | 0 | 2618 | 2450.5 | *5812.54 | *** | 257 | |||||||||
| IgG (77) | X11 | B6 | 0 | 2694.5 | 2527 | *6008.50 | *** | 324 | |||||||||
| IgG (77) | X12 | C6 | 0 | 1273 | 1105.5 | *2553.26 | *** | 275 | |||||||||
| IgG (77) | X12 | D6 | 0 | 1259.5 | 1092 | *2522.56 | *** | 244 | |||||||||
| IgG (77) | X13 | E6 | 0 | 1694 | 1526.5 | *3532.69 | *** | 267 | |||||||||
| IgG (77) | X13 | F6 | 0 | 1713.5 | 1546 | *3579.04 | *** | 222 | |||||||||
| IgG (77) | X14 | G6 | 0 | 1168 | 1000.5 | *2315.78 | *** | 308 | |||||||||
| IgG (77) | X14 | H6 | 0 | 1186 | 1018.5 | *2356.28 | *** | 226 | |||||||||
| IgG (77) | X15 | A7 | 0 | 1863 | 1695.5 | *3937.03 | *** | 302 | |||||||||
| IgG (77) | X15 | B7 | 0 | 1440.5 | 1273 | *2937.92 | *** | 264 | |||||||||
| IgG (77) | X16 | C7 | 0 | 1528.5 | 1361 | *3142.73 | *** | 280 | |||||||||
| IgG (77) | X16 | D7 | 0 | 1591 | 1423.5 | *3289.28 | *** | 245 | |||||||||
| IgG (77) | X17 | E7 | 0 | 799.5 | 632 | *1507.73 | *** | 282 | |||||||||
| IgG (77) | X17 | F7 | 0 | 774.5 | 607 | *1454.52 | *** | 226 | |||||||||
| IgG (77) | X18 | G7 | 0 | 2926.5 | 2759 | *6609.18 | *** | 204 | |||||||||
| IgG (77) | X18 | H7 | 0 | 2970 | 2802.5 | *6722.87 | *** | 265 | |||||||||
| IgG (77) | X19 | A8 | 0 | 1990.5 | 1823 | *4246.01 | *** | 256 | |||||||||
| IgG (77) | X19 | B8 | 0 | 1926 | 1758.5 | *4089.29 | *** | 290 | |||||||||
| IgG (77) | X20 | C8 | 0 | 3941 | 3773.5 | 9346.11 | 9346.11 | 233 | |||||||||
| IgG (77) | X20 | D8 | 0 | 4036.5 | 3869 | 9612.92 | 9612.92 | 216 | |||||||||
| Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature | |||||||||||||||||
| ***Value not available / --- = Designated as an outlier | |||||||||||||||||
| *Value extrapolated beyond standard range | |||||||||||||||||
| OOR = Out of Range / OOR> = Out of Range Above / OOR< = Out of Range Below | |||||||||||||||||
| Exp Conc = Expected Concentration / Obs Conc = Observed Concentration | |||||||||||||||||
| Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130% | |||||||||||||||||
| Regression Type: Logistic - 5PL | |||||||||||||||||
| Std. Curve: FI = -167.497 + (25813.5 + 167.497) / ((1 + (Conc / 76.3707)^-1.51703))^0.584385 | |||||||||||||||||
| FitProb. = 0.8682, ResVar. = 0.2405 | |||||||||||||||||
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